Gel Electrophoresis

Gel Electrophoresis Procedure We began the experiment by sealing wax the ends of the gel- molding tray with tape, and inserting the comb. We placed the tray out of the way to make authentic that it was non disturbed. We poured more or less 6mm of agarose solution into the casting tray. The gel covered solely about 1/2 the height of the combs teeth. We re intensity level outd self-aggrandising bubbles with the tip of a transfer pipette musical composition the gel was still a liquid. While waiting about hug drug minutes for the gel to solidify, we made undisputable not to move the casting tray.

After the gel solidify we unsealed the ends of the casting tray. We placed the tray in the gel loge, so that the comb was at the negative end. We poured 250ml of tris-borate-EDTA (TBE) buffer into the gel box to a take aim that covered the entire come up of the gel. We gently removed the comb, qualification sure not to pedigree the wells. The sample wells left by the comb were totally submerged. We care profusey used the pipet to load the DNA into th...If you sine qua non to get a full essay, order it on our website: OrderEssay.net

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